ser503 phospho kv3 1β Search Results


ser503 phospho kv3 1β  (PhosphoSolutions)
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PhosphoSolutions ser503 phospho kv3 1β
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Ser503 Phospho Kv3 1β, supplied by PhosphoSolutions, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ser503 phospho kv3 1β - by Bioz Stars, 2026-09
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5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of Kv3.1β and Ser503 pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation

Journal: Molecular Psychiatry

Article Title: Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action

doi: 10.1038/s41380-019-0379-3

Figure Lengend Snippet: 5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of Kv3.1β and Ser503 pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation

Article Snippet: Staining and analysis of 5-HT5A and Ser503 phospho Kv3.1β (pKv3.1β) were done for all samples at the same time using commercial antibodies against 5-HT5A (LifeSpan Biosciences, LS-A2119), Ser503 phospho Kv3.1β (Phosphosolutions, p1550-503), and PV (Sigma Aldrich, P0388).

Techniques: Transfection, Incubation, Western Blot, Activity Assay, Activation Assay, De-Phosphorylation Assay